Real-time quantitative PCR in the detection of nucleic acid blood

Objective Discussion of realtime PCR technology unpaid blood donors nucleic acid detection application effect.Methods After ALT,HBsAg,anti-HCV,anti-HIV,anti-TP,initial inspection,retest blood donors detected twice after passing blood samples,and then real-time quantitative PCR technology for nucleic acid detection.Results ELISA detection of qualified parts 242698 blood donors blood samples by realtime quantitative PCR,HBV DNA reactivity 137 cases,HCV RNA reactivity 0 cases,HIV RNA Reactivity 0 cases,the reaction rate of 0.057%.Conclusion Voluntary blood donors by nucleic acid testing work carried out,which can effectively shorten virus detection window period,to further enhance blood safety.