Covalent organic frameworks （COFs）, covalently assembled from the condensation reactions of organic building blocks, are a fascinating class of functional porous materials with two- or three-dimensional crystalline organic structures. Generally, it is preferable to use symmetric and rigid building blocks to construct highly crystalline COFs with desired topology. On the other hand, the incorporation of chiral functional moieties in the building blocks would open up new applications such as asymmetric catalysis and chiral separation. This mini review highlights the principle strategies in the design and synthesis of chiral COFs. The interesting and potential applications of these chiral COFs for asymmetric catalysis and chiral separation are also summarized. This mini review aims to provide an up-to-date advancement of chiral COFs for asymmetric catalysis and chiral separation.